Crushing and Enrichment through Separation

Enrichment of rare cancer cells through depletion of normal cells using density and flow-through, immunomagnetic cell separation.

OBJECTIVE To develop a reliable technique to enrich for rare cells in blood suspensions using only negative selection steps including a flow-through immunomagnetic cell separations system and by optimizing variables normally encountered during such enrichment processes. METHODS A human breast cancer cell line was cultivated and spiked at a ratio of 1 cancer cell to 10(5) total leukocytes in b...

Passive optical separation and enrichment of cells by size difference.

A size-selective cell sorting microfluidic device that utilizes optical force is developed. The device consists of a three-dimensional polydimethylsiloxane microstructure comprised of two crossed microchannels in a three-dimensional configuration. A line shaped focused laser beam is used for automatic size-selective cell sorting in a continuous flow environment. As yeast cells in an aqueous med...

Content Enrichment Through Dynamic Annotation

This paper describes a technique for interceding between users and the information that they browse. This facility, that we term ‘dynamic annotation’, affords a means of editing Web page content ‘on-the-fly’ between the source Web server and the requesting client. Thereby, we have a generic way of modifying the content displayed to local users by addition, removal or reorganising any informatio...

Virtual environmental enrichment through video games

Metabolic enrichment through functional gene rules

It is well known that tumors originating from the same tissue have different prognosis and sensitivity to treatments, depending on their molecular features. Over the last decade, cancer genomics consortia like the Cancer Genome Atlas (TCGA; https://cancergenome.nih.gov) have been generating thousands of cross-sectional data, spanning from genetic and epigenetic mutations to proteome profiles, f...